| Congresso Brasileiro de Microbiologia 2023 | Resumo: 985-2 | ||||
Resumo:It is increasingly possible to observe na increase in the number of research regarding the biological activities of plants, because plant extracts and essential oils are efficient in controlling the growth of various microorganisms. One of the nanomaterials most used as antimicrobials are silver nanoparticles (AgNPs), however their traditional synthesis results in the production of dangerous by products, thus increasing the demand for green synthesis alternatives. In the search for a native plant, the species Myrcia glomerata stands out, due to the lack of studies on its biological activities demonstrating its use as an alternative for the control of pathogenic bacteria. This work aimed to synthesize silver nanoparticles (AgNPs) using the ethanolic extract (EEt) of M. glomerata and evaluate the antimicrobial activity of the extract and nanoparticle (NP) against standard bacterial strains: P. mirabilis, P. aeruginosa, S. Abaetetuba, S. Enteritidis, S. Pullorum, S. Typhimurium, B. subtilis, E. faecalis, S. aureus and S. epidermidis. To obtain the extracts, the leaves of the plant were dried at 40 ºC and ground in a knife mill. Subsequently, passed through vacum filtration and rotoevaporated, for complete removal of the solvente. The green synthesis of AgNPs followed the chemical reduction methodology, which consists of the contact reaction of plant extract, ultrapure water and AgNO3 ion solution. In this work, different conditions were used (pH and synthesis time), resulting in two nanoparticles (NP1 e NP2). The antimicrobial activity was performed according to the Clinical and Laboratory Standards Institute (2017). Both the extract and the nanoparticles showed high bacteriostatic and bactericidal potential. Among the strains tested, S. aureus was the most susceptible, while E. faecalis was the most resistant. The antimicrobial activity of EEt showed MIC values ranging from 0.78-12.5 mg/mL and MBC from 0.78-50 mg/mL. As for the NPs, lower concentrations were needed to inhibit and/or cause cell death of the strains with MIC and MBC ranging from 0.026-0.053 mg/mL. Future studies may be needed to better understand the underlying mechanisms and optimize the use of the extract and the nanoparticulate as a possible tool in combating bacterial infections. Palavras-chave: antimicrobial activity, Myrcia glomerata, nanoparticles, plant extracts Agência de fomento:Fundação Araucária e UNIOESTE | |||||